Title: Welcome: DaMBIC Descriptive info: .. DaMBIC.. Login.. Links.. Olympus FV1000 MPE confocal.. Nikon - Andor Spinning Disc Confocal.. Home.. Equipment.. Booking and Fees.. Access to DaMBIC.. About DaMBIC.. Motivations.. Organization.. Principal Investigators.. Center objective.. Contact.. Fluorescence and Photonics Ph.. D.. course 2013.. Monday January 7 Friday January 11, 2013.. DaMBIC MEMPHYS, University of Southern Denmark, Odense Campus, Denmark.. Newsletter.. Please subscibe to the DaMBIC newsletter - keeping you informed about progress, events and news in bioimaging at University of Southern Denmark (No spam and easy to unsubscribe).. Click here to subscribe.. Danish Molecular Biomedical Imaging Center (DaMBIC)..
Title: Equipment: DaMBIC Descriptive info: Bioimaging Equipment.. The faculty of Natural Sciences houses equipment suitable for imaging specimens ranging from single molecules to cells and tissue.. Image modalities include atomic force microscopy, fluorescence microscopy (one- , two-photon, and spinning disc confocal, wide-field, and spinning disc confocal), and in the near future mass spectrometry imaging (MALDI imaging).. The faculty of Health Sciences houses equipment suitable for imaging specimens ranging from cells and tissue to small animals.. Image modalities include fluorescence microscopy (one- and two- photon confocal), and in vivo fluorescence and luminescence imaging.. We will also have a research scientist, whose primary job duties are to ensure that all equipment is working optimally, to train and advice external users, and if requested assist users in image acquisition and analysis.. 1.. Nikon / Andor Spinning Disc Confocal.. Motorized, inverted Nikon Ti-E microscope with Perfect Focus System equipped with Yokogawa CSU-X1 spinning disc and Andor Laser launcher with 405, 491, 514, and 561 nm laser lines.. This system has a very flexible electron-multiplied CCD camera (Andor EMCCD DU-885, 1004x1002 pixels, 8x8 µm).. This system is equipped with an Okolab microscope stage incubator for enabling live cell experiments at 37.. o.. C in 5% CO.. 2.. This system was acquired in 2010 as a component of DaMBIC.. Location: MEMPHYS - Center for Biomembrane Physics.. ... The instrument is capable of measuring laterally resolved thickness maps for ultrathin (nm) films on solid substrates in air or water.. The instrument can also characterize the dielectric properties of the film and substrate.. The instrument can be fitted with a modular Langmuir trough (Nima) to enable BAM microscopy (probe free) of Langmuir monolayer films at the air/water interface.. Location: MeMPHYS - Center for Biomembrane Physics.. Contact: Adam Cohen Simonsen, E-mail:.. adam memphys.. 4.. The Bruker UltrafleXtreme MALDI TOF-TOF tandem mass spectrometer.. We are currently establishing imaging MALDI mass spectrometry as a research tool in DAMBIC and at the Department of Biochemistry and Molecular Biology at SDU.. The Bruker UltrafleXtreme MALDI TOF-TOF tandem mass spectrometer enables peptide and protein profiling in biological specimen, as well as amino acid sequencing of peptides.. We explore a range of applications of this technology in biological and biomedical research, including imaging MS of peptides and lipids in mammalian tissues.. Contact: Ole N.. Jensen, E-mail:.. jenseno bmb.. Instrument info about MALDI-TOF/TOF mass spectrometer.. 5.. IVIS Spectrum.. The.. IVIS.. uses an optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell-trafficking and gene-expression patterns in live animals.. You have the advantage of bioluminescent and fluorescent reports across the blue to near infrared wavelength region.. Also single-view 3D-tomography is provided..
Title: Booking and Fees: DaMBIC Descriptive info: Future fees / Prices.. Autonomous access to DaMBIC equipment is limited to users who have received.. prior training.. by the DaMBIC staff.. Following completion of the training, users will gain access to the booking system on the specific equipment that they have received training on.. At that time users are free to reserve equipment time and to use the equipment at their own leisure.. From.. 2013 January 1st.. we introduce user fees to cover the future costs of IVIS and the confocal microscopes.. The user fees are differentiated according to whether ... basis of costs and estimated operating hours.. Users from the University of Southern Denmark.. and OUH associated from the Institute of Clinical Research.. With.. technician.. assistance.. DKK 359 per.. hour.. During the.. training of.. personnel.. DKK.. 253.. per.. Without.. Users outside the University of Southern Denmark.. DKK 823 per.. 823.. 365.. Booking - selected equipment.. Campus - Memphys:.. Nikon Spinning Disk Confocal.. Winsløwparken.. IVIS.. Confocal MPE.. Online Booking here.. We have changed the booking system.. Please contact us for the booking username and password.. Phone : 2478 2285.. E-mail :.. UHansen health..
Title: Access to DaMBIC: DaMBIC Descriptive info: Equipment Access:.. Access to DaMBIC equipment is available by either of two routes.. 1) Autonomous Acquisition (on select equipment).. Autonomous access to DaMBIC equipment is limited to users who have received prior training by the DaMBIC staff.. At the moment, autonomous access is only available on:.. a) A Multi photon Olympus laser scanning confocal microscope.. b) A Nikon/Andor spinning disc confocal microscope.. c) IVIS Spectrum.. 2) Assisted Acquisition / Scientific Collaboration.. In limited circumstances, additional assistance beyond mandatory training sessions is also available to use techniques available in the research groups associated to DaMBIC.. Access can be available on:.. Multiphoton excitation microscopy -based techniques such as Fluorescence lifetime Imaging (FLIM), Fluorescence correlation Spectroscopy (FCS), Raster Imaging Correlation Spectroscopy (RICS) and Second harmonic generation Imaging.. Additionally, Total Internal Reflection Fluorescence microscopy (TIRF), ROBOTICS, PET CT, Mass Spectrometry Imaging (MALDI) as well surface techniques such as Atomic Force ... (bagatolli memphys.. ).. SDU-Health, please send an email to Prof.. Henrik Ditzel (.. hditzel@memphys.. User fees:.. There are currently no user fees.. However on January 1.. st.. 2013 user fees will be instituted in order to cover minor equipment repair, such as for example replacement of light bulbs, lasers and microscope filters.. Publication policy:.. DaMBIC should be acknowledged in any publications arising from data collected on DaMBIC equipment using e.. g.. the following text :.. The bioimaging experiments reported in this paper were performed at DaMBIC, a bioimaging research core facility, at the University of Southern Denmark.. DaMBIC was established by an equipment grant from the Danish Agency for Science Technology and Innovation and by internal funding from the University of Southern Denmark.. Assisted acquisition, beyond initial training, should result in co-authorship by persons responsible for the work in addition to an acknowledgement statement as above..
Title: About DaMBIC: DaMBIC Descriptive info: Funding for the establishment of a Danish Molecular Biomedical Imaging Center (DaMBIC) at the University of Southern Denmark was granted in December 2009 from the 2009 Call for funding to Danish Research Infrastructure.. Press release.. Funding has also been provided by the Faculties of Natural Sciences and Health Sciences at the University of Southern Denmark..
Title: Motivations: DaMBIC Descriptive info: Life (and death) is dependent on complex networks of molecular interactions between proteins, lipids, DNA and RNA with remarkable specificity and diversity.. These molecular interactions are regulated at sub-cellular spatial scales, sometimes on the order of nanometers, Danish Molecular Biomedical Imaging Center and on second, or even sub-second time-scales.. This exquisite spatial and temporal regulation is lost in biochemical bulk assays and instead requires advanced bioimaging techniques with preferably single-molecule sensitivity in biological specimens ranging in size from single molecules to whole animals.. Necessary instrumentation is expensive and requires skilled operators.. Hence, such equipment is best utilized in multi-user facilities, in order to maximize societal and scientific return ... of diseases.. It is in response to these general challenges and the specific representative projects of the PIs that we propose the establishment of DaMBIC, a national biomedical imaging research center.. This initiative is driven both by the pre-existence of a broad, collective, expertise in bioimaging and by a broad common research interest and ambition for investigating biomolecular interactions over several length (from single molecules to animals) and time (from milliseconds to days) scales.. We strongly believe that this combination of broad scientific expertise and a broad common research interest provide the necessary basis for creating DaMBIC, a premier, unique, interdisciplinary, multi-user, national bioimaging research center in Denmark..
Title: Organization: DaMBIC Descriptive info: Organization.. The establishment of DaMBIC is a joint collaboration between the faculties of Natural Sciences and Health Sciences at the University of Southern Denmark in Odense.. In accordance, responsible center scientists come from both faculties and there are center locations at each faculty.. The existence of separate locations is also motivated by a strong ... specimens.. There are two main center locations, one at the MEMPHYS Center at the Natural Science faculty, and one at the Animal Research Facility at the Health Science faculty.. The directors of DaMBIC are Luis Bagatolli and Henrik Ditzel.. The daily leader in DaMBIC is Torben R.. Uhrenholt and coordinator is Ulla M.. Hansen..
Title: Principal Investigators: DaMBIC Descriptive info: The principal investigators (PIs) responsible for the establishment of DaMBIC are members of the Faculties of Natural Science (NAT) and Health Science (HEALTH) at the University of Southern Denmark (SDU), and of Odense University Hospital (OUH).. PIs are further divided at SDU-NAT within the.. Department of Physics and Chemistry.. (Mouritsen, Cohen Simonsen, Lagerholm) and the.. Department of Biochemistry and Molecular Biology.. (Bagatolli and Nørregaard Jensen), and at SDU-HEALTH within the.. Institute of Molecular Medicine.. at the.. Department of Cancer and Inflammation Research.. (Ditzel and Mollenhauer),and the.. Department of Cardiovascular and Renal Research.. (Jensen) and the.. Institute of Clinical Research.. (Kassem and Høilund-Carlsen), and at Odense University Hospital from the Departments of Oncology (Ditzel), Nuclear Medicine (Høilund-Carlsen), and Endocrinology (Kassem).. PIs include center directors, leaders and members of three Danish Centers of Excellence: the.. MEMPHYS - ... , Institute of Molecular Medicine, Department of Cardiovascular and Renal Research.. Professor.. Henrik Ditzel.. ,.. Institute of Molecular Medicine, Department of Cancer and Inflammation Research, NanoCAN - Lundbeck center for Nanomedicine.. Jan Møllenhauer.. Professor MSO.. Luis Bagatolli.. , Department of Biochemistry and Molecular Biology, MEMPHYS-Center for Biomembrane Physics, NanoCAN - Lundbeck center for Nanomedicine.. Professor Moustapha Kasseem.. , Institute of Clinical Research, Medical Endocrinology, NanoCAN - Lundbeck center for Nanomedicine.. Ole Mouritsen.. , Department of Physics and Chemistry, MEMPHYS-Center for Biomembrane Physics, NanoCAN - Lundbeck center for Nanomedicine.. Professor Ole Nørregaard Jensen.. , Department of Biochemistry and Molecular Biology, NanoCAN - Lundbeck center for Nanomedicine.. Professor Ole Skøtt, Dean of Faculty of Health Science.. Professor Poul Flemming Høilund Carlsen.. , Institute of Clinical Research, Clinical Physiology and Nuclear Medicine.. Associate Professor.. Torben R.. Uhrenholt.. ,..
Title: Center objective: DaMBIC Descriptive info: To create a multi-disciplinary state-of-the-art, open access, multi-modal molecular bioimaging imaging research center that is suitable for imaging specimens ranging from single molecules to rodents.. Incorporated DaMBIC imaging techniques for enabling molecular biomedical imaging ranging from single molecules to small rodents..